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Dr. Bartolome R. Celli, chief of pulmonary care at St. Elizabeth's Medical Center in Boston, applauded the Dutch study but called for more research.
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The vaccine consisted of 106 -irradiated CT26 or C26GM cells injected s.c. For ACT experiments, mice were vaccinated on days 14 and 7 before ACT in all four limbs with 106 -irradiated C26GM. The CD8+ T cells were negatively purified using the CD8+ isolation kit (Miltenyi Biotec), and 3 x 106 CD8+ T cells were transferred per mouse. In the 4T1-HA experiments, HA-specific CD8+ naive T cells were isolated from spleens and lymph nodes of pCL4-TCR mice.
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